Green synthesis, cytotoxicity and antimicrobial activities of some new pyrazolines, pyrimidines and naphthyridines based on 1,3-di(thien-2-yl)prop-2-en-1-one using choline chloride-urea mixture as a deep eutectic solvent

An efficient and facile green synthesis of chalcone derivatives 1a-d was achieved by treatment aromatic aldehydes with 2-acetylthiophene in the presence choline chloride-urea mixture as a deep eutectic solvent. Chalcone 1a used reactive key precursor to design series bio-active heterocycles such pyrimidine, pyrazoline, pyridine, pyridopyrimidine naphthyridine. The Structural formula were confirmed characterized their elemental analyses spectral data (IR, MS, 1H NMR, 13C NMR). In addition, synthesized evaluated for antimicrobial activities, it found that compounds 3, 8 9a exhibited potent antifungal activity comparison standard drug. Cytotoxicity against breast cancer (MCF7) screened also most showed low moderate activity. results viral screening HBV selected indicated (9b, 15), 8, (3, 6), 5 replication inhibition.